本文作者M同学，来自北京理工大学，机械工程专业，大学二年级，成绩优异，计划本科毕业后赴美读研究生，考虑到以后的就业情况，希望攻读生物医学工程相关专业研究生，转专业申请研究生，相比于本专业而言有一些困难，为了获得名校的录取，增加申请竞争力，为此报名了美国名校科研生物医学工程专业，参与选拔。参加美国名校科研，增加学术背景，开拓视野，获得真知。

选择在大二结束后的暑假参与科研，有独特的优势，学生表现优异，就成为了教授课题组的一员，以后大三、大四可以继续深入进行科研，这样会获得连续性的科研履历，更有利于美国顶尖大学研究生院的录取。

M同学非常期待此次的科研，这是他第一次来到世界顶尖名校的实验室里做科研。既期待又紧张，因为是跨专业，M同学私下阅读了很多生物医学工程相关的书籍，加强专业履历和技能背景，增加未来名校录取的竞争力。我们非常高兴地看到，学生在科研感受中写道：“我非常享受美国科研的过程，过的非常充实并且学到了很多专业相关的最前沿的知识和研究技能，也发现了很多自己需要提升的地方，更加坚信了自己未来的专业选择和人生道路。”
作为科研申请老师，我们想告诉每位参与美国科研的孩子，获得推荐信、科研证书、科研报告、科研履历，其实仅仅是美国科研的基础性收获。更为重要的是，学生通过与顶尖学者、专家的交流，树立了长期的学术目标，让学术能力伴随着生命的成长而发展，让学术赞美生命！

美国科研就是背景提升，目标就是提高本，硕，博录取质量。

科研总结

本文是学生在美国大学科研学习结束后所写的感受，并在文后附录了学生的推荐信，供学生及家长参考。

注：中文译文是编辑进行的整理，英语能力较好的学生和家长建议直接阅读英文。

Week 1 第一周

This week is the first I am working on a research project at American University. In the first week, I learned many basic skills in the lab. I learned how to use the pipette correctly. I thought it was very easy at first because I’ve used in my senior high school, but when I use it I found I was wrong. It needs to be accurate and it is very difficult especially use the pipette to have 1ul solution. I even got 0.5ul by mistakes. By trying it for lots of times, I can use it much well. Then I learn to mix a buffer A for HPLC. I know the composition of it and calculate the mass of each one the I dissolve each and use the PH detector to adjust the PH of it to 7. It’s the first time I have to make a reagent and it helps me know we need to think thoroughly about how much of each component should we add.

这周是我在美国大学进行的第一个研究项目。第一周，我在实验室学到了很多基本技能，我学会了如何正确使用吸液管。一开始我觉得这很容易，因为我在高中就用过，但当我用的时候，我发现我错了。它需要准确，特别是使用移液管获得1ul溶液非常困难。我甚至因为错误而得到了0.5ul。通过多次尝试，我可以很好地使用它。然后我学习混合一种缓冲液A用于高效液相色谱。我知道它的成分，并计算出每一种物质的质量，我溶解每一种物质，然后用酸碱度检测器把它的酸碱度调整到7。这是我第一次做试剂，这有助于我知道我们需要仔细考虑每种成分应该添加多少。

Then came the second day. I make the standard mixture for the HPLC. As I got the instruction, I think it’s a quite difficult one. First, I need to dissolve all the compounds into 2ml stock solution. I need to consider the purity of each compound. It really needs my patience because I need to fetch only 2mg and it needs to be very accurate. After I finished it, I need to calculate the volume needed for each one to make the mixture. I used my pen and calculate by hand at first, but my professor taught me to use excel to do the calculation, I soon learned how to do it and found it much more convenient. In the end, I adjust pH around 7 with pH paper, and that’s an easy task.

第二天来了。我为HPLC制作标准混合物。当我得到指示时，我认为这是一个非常困难的指令。首先，我需要将所有化合物溶解到2ml储备溶液中。我需要考虑每种化合物的纯度。它真的需要我的耐心，因为我只需要获取2毫克，它需要非常准确。完成之后，我需要计算每个混合物所需的体积。我一开始用笔和手工计算，但是我的教授教我使用excel进行计算，我很快就学会了如何去做，发现它更方便。最后，我用pH试纸调节pH值约为7，这是一项简单的任务。

Before the third day, I used Google to search how does the HPLC work. And I know the function of each part of HPLC. One that day, I try to operate the HPLC. It was a little nervous because I always heard people saying that HPLC is difficult. However, when my professor gave me the guidance of it and show me how to do that, it became much easier. I follow the guidance and prime the pumps and then purge them using methanol, water and then buffers. Then I run the However standard mixture I made yesterday for the HPLC to analyze it. I firstly used the 50ul standard mixture. But after I had my lunch, there is something wrong with the computer and the data loss. I had to do it again and wait for 2 hours again. That’s frustrating. Finally, I successfully got the result below. Then I try 100ul as well then go the same result. It means that my standard mixture is the correct one.

在第三天之前，我用谷歌搜索高效液相色谱是如何工作的。我知道高效液相色谱各部分的功能。一天，我试着操作高效液相色谱法。有点紧张，因为我经常听到人们说高效液相色谱法很难。然而，当我的教授给我指导并告诉我如何做的时候，事情变得简单多了。我遵循指导，启动泵，然后用甲醇、水和缓冲器进行清洗。然后我运行昨天为高效液相色谱分析而制备的标准混合物。我第一次使用50ul标准混合物。但是在我吃过午饭后，电脑出了点问题，数据丢失了。我得再做一次，再等两个小时。真令人沮丧。最后，我成功地得到了下面的结果。然后我也尝试100ul，然后得到相同的结果。这意味着我的标准混合物是正确的。

I feel really excited about it’s the first time that I operate HPLC. Through the first week, I find it’s very interesting to do a project in the lab. I can learn much more things than in traditional classes. And I also know I should be more patient. What’s more, the most important thing I learned from the first week is the powerful function of excel. And I will learn to use excel more fluently in my daily study.

我感到非常兴奋，因为这是我第一次操作高效液相色谱。在第一个星期里，我发现在实验室里做一个项目很有趣。比起传统的课程，我能学到更多的东西。我也知道我应该更有耐心。而且，我从第一周学到的最重要的是Excel的强大功能。我将学会在日常学习中更流利地使用Excel。

Week 2 第二周

This week is the second week of doing research at American University.This week I continued my HPLC job. Things have been much more similar to me than the first week. Everything turns great this week. On the first day, I finish my first sample and protein. I followed the instruction provided by my professor. I firstly made some preparation such as having the tubes ready and preparing other compounds. Then I completed the sample1 step by step with the help of my professor. It’s the first time for me to see liquid nitrogen, and that’s really fantastic. Meanwhile, I also did the 20ul and 100ul injection of my standard mixture. Then on the second day, I continued my HPLC project. I injected 40ul and 60ul standard mixture. After several days’ practice, I can operate the machine much faster. At the same time, I made two samples of the tissue. It’s the major task I could do in this project. It’s quite difficult for me because I need to use the liquid nitrogen and I need to be really patient for it has some danger. Finally, I made it following the instruction. I am really delighted as I finish the task.

这周是在美国大学做研究的第二周，这周我继续我的高效液相色谱工作。事情比第一周更像我。这周一切都变好了。第一天，我完成了我的第一个样品和蛋白质。我听从教授的指示。我首先做了一些准备工作，比如准备好管子和准备其他化合物。然后，在我的教授的帮助下，我一步一步地完成了这个样本。这是我第一次看到液氮，真是太棒了。同时，我还注射了20ul和100ul的标准混合物。第二天，我继续我的高效液相色谱项目。我注射了40ul和60ul的标准混合物。经过几天的练习，我能更快地操作机器。同时，我做了两个组织样本。这是我在这个项目中可以做的主要任务。这对我来说相当困难，因为我需要使用液氮，我需要非常耐心，因为它有一些危险。最后，我按照指示做了它。我很高兴完成这项任务。

As the last day for this week came, I carried on my job for making samples 4,5,6,7 of the two groups. Meanwhile, I do my injections one by one. It becomes much easier to finish my job than I firstly finish the task. All the result seem great. In the end, I finished my job faster than we have thought. Therefore, the professor taught me how to analyse the data of the HPLC.

当这周的最后一天到来时，我继续我的工作，在两组中制作4，5，6，7个样品。同时我一个接一个地注射。完成工作比我先完成任务容易得多。所有的结果都很好。最后，我比我们想象的更快地完成了我的工作。因此，教授教我如何分析高效液相色谱数据。

In this week the operation in the experiments became much and much easier for me. Because I know each step of the experiments. For example, when I finish the samples, all the steps are same for each sample. As I have finished more and more samples, I can behave much better and much faster. But I also know that speed is not what we need in the end, we need the accuracy. One standard mixture’s result seems strange after the injection. I think I may make some mistakes in the process. From this I realize that we should spend more time considering about how to make the correct result instead of how to do the experiments faster. I will mind that in my study in the future.

在这周，实验的操作对我来说变得越来越简单。因为我知道实验的每一步。例如，当我完成样本时，每个样本的所有步骤都是相同的。因为我已经完成了越来越多的样品，我可以表现得更好更快。但我也知道速度不是我们最终需要的，我们需要的是准确度。注射后一种标准混合物的结果似乎很奇怪。我想在这个过程中我可能会犯一些错误。由此我认识到，我们应该花更多的时间来考虑如何得到正确的结果，而不是如何更快地进行实验。我以后在学习时会介意的。

Week3 第三周

This week is the third week I have been studying at American University. This week, in general, I finish preparing the samples, continue injecting the samples and starting the Lowry Protein assay. Although something was not so satisfactory, I finally finish all the tasks. The first day is another day for making and injecting samples. I tried to operate the machine without the instruction to check whether I have mastered the steps correctly. At first, it bothers me a lot, I need to recall what I did last time and double-check it to ensure I don’t make a mistake. Gradually it turns much easier for me as I carry on my experiments. And everything shows great in the end. I make another 4 samples and inject 3 of them. Meanwhile, I also make some data analyses in my spare time. I think I master the skills well after two-weeks practice.

这周是我在美国大学学习的第三周。一般来说，这周我完成了样品的准备，继续注射样品，开始了低蛋白分析。虽然有些事情不太令人满意，但我最终完成了所有的任务，第一天又是制作和注射样本的另一天。我试着在没有指示的情况下操作机器，以检查我是否正确掌握了这些步骤。一开始我很烦恼，我需要回忆我上次做的事情，然后再检查一遍，以确保我不会犯错误。随着实验的进行，它逐渐变得对我来说容易多了。最后一切都很好。我又做了4个样品，注射了3个。同时，我也在业余时间做了一些数据分析。我想经过两周的练习，我掌握得很好。

On the second day I make the last three samples and finally finish my task for finishing 14 samples. I’m really proud of that because it’s my first time to do things like that. I find a lot of interests during I make those. Then I continue my injections as usual. Meanwhile, I finish my data analyses of the standard mixture. However, there is something wrong with that. Most of my standard mixtures only show 8 peaks instead of 9. It really confused me a lot. I dissolved 9 compounds in my standard mixture but why only show 8 peaks. Then my professor helps me with that. We check the nine compounds I made in the first day. And we find that the reason is that my Adenosine probably doesn’t dissolve in the resolution, that’s the missing peak. I think I might use the pure NH4OH to dissolve it. Then professor gives me his Adenosine data for me to calculate. I will put this in my mind. And I finally know the reason professor asked me to keep the each solution of every compounds.

第二天，我做了最后三个样本，最后完成了14个样本的任务。我真的为此感到骄傲，因为这是我第一次这样做。我在制作过程中发现了很多兴趣。然后像往常一样继续注射。同时，完成了标准混合物的数据分析。但是，这有点问题。我的大多数标准混合物只显示8个峰值而不是9个。我真的很困惑。我在标准混合物中溶解了9种化合物，但为什么只有8个峰。然后我的教授帮我解决这个问题。我们检查了我第一天制造的九种化合物。我们发现原因是我的腺苷可能不会溶解在分辨率中，这就是缺失的峰值。我想我可以用纯NH4OH来溶解它。然后教授给我他的腺苷数据让我计算。我会记住这一点。我终于知道教授让我保留每种化合物的每一种溶液的原因。

Week 4 第四周

This week is the third week I have been studying at American University. And I must say time passed so quickly and I really enjoy this experience because I think I like using my hands to do some experiments. And this week I finished all my sample injections and analyzed all the data and make the tables of them.

本周是我在美国大学学习的第三周。而且我必须说时间过得如此之快，我真的很喜欢这种体验，因为我觉得我喜欢用手来做一些实验。本周我完成了所有样品注射并分析了所有数据并制作了表格。

On the first day, A good news for today is that the HPLC’s problem has been solved, so I carry on my injections. And I have finished the last four samples and successfully get the result. Then comes the data analyses. Professor introduces a new software called Graphpad prism to me. That is really powerful and can produce the curves according to the data. I soon learn to operate it. I’m really glad to master another useful tool for analyzing the data.

在第一天，今天的好消息是HPLC的问题已经解决，所以我继续我的注射。我已完成最后四个样本并成功获得结果。然后是数据分析。教授向我介绍了一款名为Graphpad prism的新软件。这非常强大，可以根据数据生成曲线。我很快学会了操作它。我很高兴掌握另一种分析数据的有用工具。

Then came the second day and sadly my last day for experiments. Professor He then introduced the method to figure out each peak stands for which composition. I soon got it and started to analyse all the samples. After one-morning’s effort, I finally got all the data in an excel. And professor showed me how to use Graphpad prism to deal with the data. And then I got the graphs of them. However, something wrong happened. Some of my data failed to show the correct trend and much larger than others. As I looked back on my experiment, I thought there was something wrong with my protein assay. It was really frustrated and disappointed. But professor He told me on the first day that error is not a bad thing and you should learn from the error. I think this is very meaningful and I think I should be more patient to do anything so that I could make less mistakes.

第二天来了，可悲的是我最后一天的实验。何教授然后介绍了找出每个峰值代表哪种成分的方法。我很快得到它并开始分析所有样品。经过一个上午的努力，我终于获得了excel中的所有数据。教授向我展示了如何使用Graphpad棱镜来处理数据。然后我得到了他们的图表。但是，发生了一些错误。我的一些数据未能显示正确的趋势，并且比其他数据大得多。当我回顾我的实验时，我认为我的蛋白质分析有问题。真的很沮丧和失望。但是教授在第一天就告诉我错误不是坏事，你应该从错误中吸取教训。我认为这是非常有意义的，我认为我应该更耐心地做任何事情，以便减少错误。

Then my internship ends. I think I do gained a lot from this experience. I know the basic process and methods of research in the US and I realize how precise is required in any research, which need to be really patient. Also, I have made a lot of friends during this experience and know a lot about city. What’s most important to me is that I have know more about Medical area and I can have more choices when I decide my major in the future after graduation.

I really enjoy this experience. I’m very grateful for professor He to give me a chance to have this experience and give me very patient instruction.

然后我的实习结束了。我想我从这次经历中获得了很多。我知道美国的基本过程和研究方法，并且我意识到在任何需要真正耐心的研究中需要多么精确。此外，我在这次经历中结识了很多朋友，并且对城市了解很多。对我来说最重要的是，我对医学领域有了更多的了解，毕业后我决定将来的专业可以有更多的选择。

我真的很喜欢这种体验。我非常感谢教授让我有机会获得这种经验并给予我非常耐心的指导

科研报告 （节选）

科研学习中，在美国教授的辅导下，M学生完成的专业科研报告（节选）

推荐信（节选）

First, I have been repeatedly impressed by his outstanding research ability. He was both driven and teachable, willing to invest considerable time and energy into his work and open to receive instructions to refine his skills. He is a quick learner, able to readily assimilate the goals and methods of the tasks he was assigned to. For example, within a short period, he mastered the correct usage of the pipette, learned to prepare a solution named buffer A for HPLC, familiarized himself with the functions of HPLC, and acquired the skills of processing data with Excel and Graphpad prism. Besides, he applied himself diligently and with enthusiasm throughout the whole research program by flexibly practicing what he had gained in the first stage of our research. I believe that he has made significant progress at American University, and the final report with logical structure and vivid graphs was a strong proof of his harvest.

首先，我对他杰出的研究能力印象深刻。他既有动力，又有教益，愿意在工作中投入大量的时间和精力，愿意接受指导来提高他的技能。他学得很快，能够很容易地吸收他所分配任务的目标和方法。例如，在很短的时间内，他掌握了移液管的正确使用方法，学会了制备高效液相色谱用缓冲液A，熟悉了高效液相色谱的功能，掌握了用Excel和graphpad prism处理数据的技巧。此外，他在整个研究项目中勤奋而热情地运用自己，灵活地实践他在我们研究的第一阶段所获得的成果。我相信他在美国大学科研期间取得了显著的进步，最终的报告用逻辑结构和生动的图表证明了他的收获。

Second, I appreciate his positive attitude towards research. It has been clear to me that he enjoys research a great deal. He often used the word “interesting” to describe his experiment, and considered it an awesome experience when firstly applied liquid nitrogen in his experiment. When encountered with problems during the research process, he never complained; instead, he positively attempted to get through it by himself first and provided multiple solutions. Later, he would discuss with me and revise his solutions accordingly. He thought mistakes were not bad, while lessons drawn from mistakes were most valuable. I think such a proactive research attitude is the cornerstone for him to be an excellent researcher in the near future.

第二，我欣赏他对研究的积极态度。我很清楚他很喜欢研究。他经常用“有趣”这个词来描述他的实验，并认为这是一个了不起的经验时，首次应用液氮在他的实验。在研究过程中遇到问题时，他从不抱怨；相反，他积极尝试自己先解决问题，并提供了多种解决方案。稍后，他会和我讨论并相应地修改他的解决方案。他认为错误并不坏，而从错误中吸取的教训是最有价值的。我认为这种积极主动的研究态度是他在不久的将来成为一名优秀研究员的。

选拔流程

1.提交报名表

2.科研组择优面试

3.面试通过后，发送录取确认书

4.协调机票、接机、住宿；

5.赴美开始科研

6.获得导师推荐信，科研证书，丰富的CV、PS履历